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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become noticed in several tumors, which results in chromosome instability, and therefore may perhaps lead to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important part in endorsing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already explained. Two further splice variants were being claimed but in-depth nucleotide sequence information and facts is just not but readily available.

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Blocking peptides are peptides that bind especially into the concentrate on antibody and block antibody binding. These peptide usually contains the epitope regarded because of the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to ensure the absolute best benefits are...

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This guideline outlines even further doable optimization of mobile and immunoassay parameters to make sure the very best benefits are...

Antibodies certain to the blocking peptide not bind on the epitope within the concentrate on protein. By comparing the staining in the blocked antibody vs . the antibody on your own, you can see which staining is specific.

Target Information and facts Cyclin E belongs on the really conserved cyclin family, whose members are characterized by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation styles which lead to your temporal coordination of each and every mitotic occasion. Cyclin E kinds a complex with and features to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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